stat5 in1 Search Results


gmec  (MedChemExpress)
95
MedChemExpress gmec
Figure 1. miR-380-3p targets 3’UTR of αS1-casein gene. (A) αS1-Casein gene and miR-380-3p abundance in goat mammary gland of different lacta tion stages. Mammary gland samples were obtained from Xinong Saanen dairy goats (n = 5) during early, peak, middle and end lactation stages. Values were normalized to early lactation stage. (B) Predicted binding site of miR-380-3p in αS1-casein 3’UTR and construction of psiCHECK-2 dual-lu ciferase reporter vectors. psi-WT-αS1-casein represents the wildtype sequence vector; psi-MUT-αS1-casein represents the mutation (red bases) vec tor. (C) miR-380-3p expression was measured by qRT-PCR. <t>GMEC</t> were treated with mimic NC or miR-380-3p mimic (50 nM) for 48 h. (D) 3’UTR activity of αS1-casein was measured by luciferase assay. Cells were co-treated with dual-luciferase vector and miR-380-3p mimic (50 nM) for 48 h. Data are presented as mean±standard error of the mean. UTR, untranslated region; qRT-PCR, quantitative real-time polymerase chain reaction; GMEC, goat mammary epithelial cells; NC, negative control.
Gmec, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stat5+in1/STAT5-IN-1/pm37170511-69-1-12
Average 95 stars, based on 1 article reviews
gmec - by Bioz Stars, 2026-10
95/100 stars
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stat5-in-1  (TargetMol)
N/A
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s6784  (Selleck Chemicals)
93
Selleck Chemicals s6784
Figure 1. miR-380-3p targets 3’UTR of αS1-casein gene. (A) αS1-Casein gene and miR-380-3p abundance in goat mammary gland of different lacta tion stages. Mammary gland samples were obtained from Xinong Saanen dairy goats (n = 5) during early, peak, middle and end lactation stages. Values were normalized to early lactation stage. (B) Predicted binding site of miR-380-3p in αS1-casein 3’UTR and construction of psiCHECK-2 dual-lu ciferase reporter vectors. psi-WT-αS1-casein represents the wildtype sequence vector; psi-MUT-αS1-casein represents the mutation (red bases) vec tor. (C) miR-380-3p expression was measured by qRT-PCR. <t>GMEC</t> were treated with mimic NC or miR-380-3p mimic (50 nM) for 48 h. (D) 3’UTR activity of αS1-casein was measured by luciferase assay. Cells were co-treated with dual-luciferase vector and miR-380-3p mimic (50 nM) for 48 h. Data are presented as mean±standard error of the mean. UTR, untranslated region; qRT-PCR, quantitative real-time polymerase chain reaction; GMEC, goat mammary epithelial cells; NC, negative control.
S6784, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stat5+in1/STAT5-IN-1/10__1172_slash_jci160790-266-13-16
Average 93 stars, based on 1 article reviews
s6784 - by Bioz Stars, 2026-10
93/100 stars
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stat5-in-1  (BOC Sciences)
N/A
STAT5-IN-1, a cell-permeable nonpeptidic nicotinoyl hydrazone compound, is a selective STAT5 inhibitor with an IC50 of 47 μM for STAT5β isoform with much less effect towards the SH2 domain of STAT1, STAT3, or Lck (IC50>500
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Image Search Results


Figure 1. miR-380-3p targets 3’UTR of αS1-casein gene. (A) αS1-Casein gene and miR-380-3p abundance in goat mammary gland of different lacta tion stages. Mammary gland samples were obtained from Xinong Saanen dairy goats (n = 5) during early, peak, middle and end lactation stages. Values were normalized to early lactation stage. (B) Predicted binding site of miR-380-3p in αS1-casein 3’UTR and construction of psiCHECK-2 dual-lu ciferase reporter vectors. psi-WT-αS1-casein represents the wildtype sequence vector; psi-MUT-αS1-casein represents the mutation (red bases) vec tor. (C) miR-380-3p expression was measured by qRT-PCR. GMEC were treated with mimic NC or miR-380-3p mimic (50 nM) for 48 h. (D) 3’UTR activity of αS1-casein was measured by luciferase assay. Cells were co-treated with dual-luciferase vector and miR-380-3p mimic (50 nM) for 48 h. Data are presented as mean±standard error of the mean. UTR, untranslated region; qRT-PCR, quantitative real-time polymerase chain reaction; GMEC, goat mammary epithelial cells; NC, negative control.

Journal: Animal bioscience

Article Title: miR-380-3p promotes β-casein expression by targeting αS1-casein in goat mammary epithelial cells.

doi: 10.5713/ab.23.0007

Figure Lengend Snippet: Figure 1. miR-380-3p targets 3’UTR of αS1-casein gene. (A) αS1-Casein gene and miR-380-3p abundance in goat mammary gland of different lacta tion stages. Mammary gland samples were obtained from Xinong Saanen dairy goats (n = 5) during early, peak, middle and end lactation stages. Values were normalized to early lactation stage. (B) Predicted binding site of miR-380-3p in αS1-casein 3’UTR and construction of psiCHECK-2 dual-lu ciferase reporter vectors. psi-WT-αS1-casein represents the wildtype sequence vector; psi-MUT-αS1-casein represents the mutation (red bases) vec tor. (C) miR-380-3p expression was measured by qRT-PCR. GMEC were treated with mimic NC or miR-380-3p mimic (50 nM) for 48 h. (D) 3’UTR activity of αS1-casein was measured by luciferase assay. Cells were co-treated with dual-luciferase vector and miR-380-3p mimic (50 nM) for 48 h. Data are presented as mean±standard error of the mean. UTR, untranslated region; qRT-PCR, quantitative real-time polymerase chain reaction; GMEC, goat mammary epithelial cells; NC, negative control.

Article Snippet: Also, GMEC were treated with STAT5 inhibitor STAT5-IN-1 (50 μM, CAS 285986-31-4; MedChemExpress, NJ, USA) followed by miR-380-3p mimic (50 nM) and psi-WT-β-Casein cotreatment.

Techniques: Binding Assay, Sequencing, Plasmid Preparation, Mutagenesis, Expressing, Quantitative RT-PCR, Activity Assay, Luciferase, Real-time Polymerase Chain Reaction, Negative Control

Figure 6. The proposed model regarding the role and regulation of miR-380-3p in goat mammary epithelial cells. miR-380-3p inhibits αS1-casein expression by targeting 3’UTR of αS1-casein gene. Then, miR-380-3p increases β-casein expression through αS1-casein/STAT5 axis. Moreover, miR-380-3p promotes β-casein transcription by the binding of STAT5 to β-casein gene promoter region. STAT5a, signal transducer and activator of transcription 5a.

Journal: Animal bioscience

Article Title: miR-380-3p promotes β-casein expression by targeting αS1-casein in goat mammary epithelial cells.

doi: 10.5713/ab.23.0007

Figure Lengend Snippet: Figure 6. The proposed model regarding the role and regulation of miR-380-3p in goat mammary epithelial cells. miR-380-3p inhibits αS1-casein expression by targeting 3’UTR of αS1-casein gene. Then, miR-380-3p increases β-casein expression through αS1-casein/STAT5 axis. Moreover, miR-380-3p promotes β-casein transcription by the binding of STAT5 to β-casein gene promoter region. STAT5a, signal transducer and activator of transcription 5a.

Article Snippet: Also, GMEC were treated with STAT5 inhibitor STAT5-IN-1 (50 μM, CAS 285986-31-4; MedChemExpress, NJ, USA) followed by miR-380-3p mimic (50 nM) and psi-WT-β-Casein cotreatment.

Techniques: Expressing, Binding Assay